Susceptibilidade In Vitro De Fungos Melanizados Isolados Da Carnaúba (Copernicia Prunifera) À Anfotericina B

Abstract

Black fungi can be found in different parts of plants, in general, this association promotes contamination in humans who come into contact with these plants. Carnauba (Copernicia prunifera) is a palm tree of great cultural and economic value,

11 which has the most diverse applications. Amphotericin B (AmB) is a drug with a broad spectrum of action, considered the gold standard in the treatment of most systemic mycoses, although its use is limited due to the induction of side effects. This pioneering study aimed to evaluate, through in vitro assays, the susceptibility of black fungi isolated from carnauba to AmB. Fungal susceptibility testing was performed in accordance with Clinical and Laboratory Standards Institute document M38-A2. The results showed that AmB inhibited the growth of 50% of the fungi tested. The isolates that demonstrate sensitivity presented Minimum Inhibitory Concentration (MIC) between 0.125μg/mL and 1μg/mL. It was also observed that three fungi showed MIC higher than the maximum concentration of AmB used (>16μg/mL), and two isolates were inhibited, but at high doses. Resistant isolates were found, which indicates the importance of analyzing the susceptibility of these fungi before starting a treatment. Therefore, the presence of black fungi in carnauba and resistant strains justify the importance of making people who work with this plant aware of the care they must follow to avoid contamination by these microorganisms, since the diseases that can be caused by these fungi have great significance for public health.