Extração de DNA de Phaseolus lunatus L. (feijão-fava) mediante diferentes tratamentos de conservação da folha

Abstract

The lima bean (Phaseolus lunatus L.) is a legume produced worldwide, known for its resilience to semi-arid climates. The isolation of its DNA aims to enhance understanding and research into its genome load, facilitating its improvement and promoting and strengthening its cultivation. This study aims to optimize the preservation of Phaseolus lunatus L. leaves to achieve high-quality DNA extraction using the protocol outlined by Dellaporta et al. (1983), employing both dried and fresh leaves. In spectrophotometry that aims to measure the purity of the sample, indicating how contaminated the sample is according to the wavelength, there was no result outside the base parameter, when comparing the study objects of the extraction carried out with the dry leaf tissue, we obtained- a greater number of results, within the parameters suggested by the authors, when correlated with freshness. When analyzing the parameter 1.8 to 2.2 at wavelength 260/280, where in the fresh leaf tissue sample there was a sample that exceeded the parameter used as base. Regarding the 260/230 wavelength ratio, dried leaf tissue extraction mostly met the 1.6-1.9 criterion, with only two exceptions, whereas fresh leaves showed six samples outside this range. Therefore, preserving DNA with dried leaf tissue is justified as the most promising method for Phaseolus lunatus L. DNA extraction.