Fluconazol e anfotericina b frente a candida spp. Isoladas de regiões urbanas e preservadas do litoral Piauiense

Abstract

Candida spp. are part of the human microbiota and are widely distributed in the environment. The research of these microorganisms in studies related to the sanitary quality of coastal environments is becoming more and more frequent. Since amphotericin B and fluconazole are widely applied in the treatment of candidiasis, the present work aimed to characterize Candida spp. regarding the in vitro susceptibility profile to these antifungals. Such isolates were obtained from samples of water and soil collected from urbanized and preserved points of 5 beaches on the Piauí coast and Delta do Parnaíba. Susceptibility tests were performed using the broth microdilution technique (CLSI document M27-A3). The presumptive identification of species of the genus Candida was performed using the CHROMAgarTM culture medium, through which 18 isolates were characterized as Candida krusei and 3 as Candida spp. The location from which the largest number of yeasts was isolated was Delta do Parnaíba. A total of 17 isolates were resistant to fluconazole and 2 to amphotericin B. All isolates resistant to amphotericin B also showed resistance to fluconazole. All soil samples were known to be resistant to fluconazole, and of this total, about 84% were isolates of Candida krusei, which is naturally resistant to fluconazole. Approximately 81% of yeasts from urban environments were resistant to at least one tested drug. In view of what was observed, it is suggested that other studies be carried out to continue monitoring the pathogenic yeasts present in these environments.