Atividade antinflamatória de nanopartícula de prata estabilizada com colágeno e goma do cajueiro (anacardium ocidentale l.)

Abstract

Silver nanoparticles (AgNP) have emerged as an important class of nanomaterials with diverse medical applications. The interest in conducting research using proteins and silver nanoparticles (AgNPs) is broad since the anti-inflammatory activity of these specific types of nanoscale clusters is unknown. The objective of this study was to describe the effect of intraperitoneal administration of silver nanoparticles stabilized with collagen and cashew gum in experimental models of paw edema and peritonitis induced by the administration of carrageenan. The potential of inhibition of paw edema of a silver nanoparticle at doses of 1ml / kg, 2ml / kg and 3ml / kg applied intraperitoneally and its action on the enzyme myeloperoxidase activity was analyzed. Oxidative stress and anti-inflammatory activity were also evaluated by the determination of reduced glutathione (GSH), nitrate and nitrite, superoxide dismutase in peritonitis. The results showed that the silver nanoparticle stabilized with collagen and cashew nut at a dose of 3 ml / kg had anti-inflammatory potential by inhibiting the formation of edema by 70% during third hour of experiment. The compound also demonstrated to reduce the activity of the enzyme myeloperoxidase significantly when compared to the control group. The antioxidant potential of the nanoparticle is demonstrated by the reduction of nitrite levels and maintenance of GSH and SOD levels when compared to the carrageenan group. It is concluded that the silver nanoparticle stabilized with collagen and cashew gum has an anti-inflammatory potential, through the inhibition of paw edema and neutrophil migration and antioxidant potential through the maintenance of GSH and SOD levels and decrease of Nitrite levels.