Efeito antiinflamatório e antioxidante de nanopartícula de prata estabilizada com colágeno e goma do cajueiro (anacardium occidentale l.)

Abstract

The discovery of natural nanomaterials has aroused interest both in research and in the drug market, with silver nanoparticles being the most used in the research environment due to their numerous applications. These nanoparticles may be associated with different compounds in order to produce a potential effect on a living organism. Therefore, the present study aims to test the antiinflammatory and antioxidant potential of a silver nanoparticle stabilized with collagen and cashew gum by topical application in experimental models of paw edema and peritonitis. The antiedematogenic potential of a silver nanoparticle at 25 μl, 50 μl and 75 μl applied topically, action on carrageenan edema, best dose on inflammatory mediators (histamine, prostaglandin E2 and 48/80), its antimicrobial effect was analyzed. action on neutrophil migration and vascular permeability. The action against oxidative stress was also evaluated by the determination of reduced glutathione (GSH), nitrite, superoxide dismutase (SOD) in peritonitis only at a dose of 75 ul. The results showed that the silver nanoparticle stabilized with collagen and cashew gum at a dose of 75 ul has antiinflammatory and antiedematogenesis potential by inhibiting the inflammatory infiltrate during the peak of carrageenan edema and inflammatory mediators. The compound has also been shown to significantly reduce myeloperoxidase enzyme activity when compared to the control group, as well as vascular permeability. The antioxidant potential of nanoparticle is demonstrated by the reduction of nitrite levels and maintenance of GSH and SOD levels when compared to the carrageenan group. In conclusion, these data show that silver nanoparticle, collagen and cashew gum reduces the inflammatory response by inhibiting vascular and cellular events, modulating neutrophil migration, inhibiting proinflammatory cytokine production and reducing oxidative stress.