Quartenização da goma do angico (anadenanthera colubrina var. cebil (Griseb.) Altschul): avaliação da atividade antimicrobiana.

Abstract

The angico of the species Anadenanthera colubrina var. Cebil (Griseb.) Altschul is found in several regions of the country, mainly in the cerrado and produces exudate whose composition presents large amount of polysaccharides (Paula, Budd e Rodrigues, 1997). The chemical modifications in the polysaccharides can be important tools to obtain new antimicrobial agentes (Coutinho, Muzitano e Costa, 2008). The objective of this work was to synthesize from the angico gum cationic derivatives as well as to evaluate the effect of angico gum and its derivatives on bacteria of the genus Staphylococcus. The modification of the gum structure was verified using infrared spectroscopy by the appearance of bands around 1470 cm -1 attributed to the -CH bond of the quaternary ammonium and elemental analysis measures confirmed the increase of percentage of nitrogen for well quaternized gum such as the most positive zeta potential values. The biocompatibility on erythrocytes was verified by hemolysis test.The best results was found for the derivates GAQ-A3 and GAQ-B with bactericidal effect of S. epidermidis ATCC 12228, S. aureus ATCC 29213 and S. aureus MRSA 43300 (GAQ-A3: MIC-MBM = 125-125 μg / mL, 250-250 μg / mL and 250-250 μg / mL, respectively and GAQ- B: MIC-CBM = 62.5-62.5 μg / mL, 250-250 μg / mL and 250-250 μg / mL, respectively). Biocompatibility and antiesthetiococcal activity indicated that the derivatives are promising as an antibacterial agent for biomedical applications.