Quaternização e acetilação da goma do cajueiro: efeito do posicionamento reacional nas propriedades físico- químicas, hemocompatibilidade e atividade antibacteriana

Abstract

Cashew gum (Anacardium occidentale L.) (CG) is a natural anionic heteropolysaccharide with potential in pharmaceutical, food and biotechnological applications. The acetylated results show amphiphilic properties used for the synthesis of drug nanoparticles. While quaternization improves solubility, it has antimicrobial activity. Therefore, the objective of this work is to verify the effect of the chemicals presented by the quaternization together, on the physical properties. Aby da had CCHPTA, NaOH and acetic anhydride as reagents. Four different programming routes were offered, where two samples were modified with one order of acetylation-combined and two combinations of differentiated acetylation generating 4 different combinations. The characterization was performed through infrared spectroscopy, molar mass, solubility, elemental analysis, nuclear magnetic resonance (NMR) and atomic force microscopy (AFM). Hemocompatibility and antibacterial activity assays were performed by the contact method. Through infrared spectroscopy it is possible to confirm the modification of the proposals, observing the location of the acetyl functional groups quaternary ammonium spectroscopy and the carbonyl group of the acetyl group. The NMR confirmed references to acetyl groups between 2.8 and 5 ppm and at 3.1 ppm attributed to the N, N, N - trimethyl of the EPTAC inserted in the chain. The increase in the percentage of carbon, hydrogen and is directly related to the degree of substitution The molar mass of the results increased

compared to CG. A of the groups developed a good solubility. A possible self- organization was observed by AFM, and also particles of larger sizes compared to

CGQ and CGA. As compatible blood, when compared to the control group. Through the test of S. aureus and E. coli bacteria it was possible to observe changes in the antibacterial on the species S. aureus and E. coli when the contact with the activity of CG, CGA and CGQ was performed. When performing an insertion of the two functional groups with different characteristics, it was possible to observe that an approach of new polymer chains improved the organizational characteristics of their particles.