http://dspace.ufdpar.edu.br/jspui/handle/prefix/205 2026-04-07T06:45:34Z 2026-04-07T06:45:34Z Silva, Maisa Gomes http://dspace.ufdpar.edu.br/jspui/handle/prefix/659 2025-07-30T18:59:08Z 2019-11-04T00:00:00Z

Título: Efeitos sobre perfil antioxidante e reatividade vascular induzidos por uma manteiga obtidas das sementes de Platonia insignis Mart. em modelo de hipertensão L-NAME em ratas Autor(es): Silva, Maisa Gomes Primeiro Orientador: Arcanjo, Daniel Dias Rufino Abstract: Cardiovascular diseases are one of the leading causes of death worldwide. In this context, the use of natural products has been presented as an important source of bioproducts with potential therapeutic applications. In the search for biologically active compounds of the Clusiaceae family, Platonia insignis Mart., Popularly known as “bacuri”, has shown vasorelaxant, antioxidant and hypotensive effects in vivo, showing its promising application in the development of products for the treatment of cardiovascular diseases involving oxidative stress. Thus, the objective of this work is to investigate the toxicological, antioxidant and vasorelaxing effects of an industrialized butter obtained from Platonia insignis Mart seeds. (MIB), through in vitro and in vivo assays, for potential application in the treatment of hypertension. Cytotoxic activity was initially performed by MTT assay on murine macrophages and sheep blood erythrocytes. In the present study, a reduction in cell viability was observed by assessing concentration-dependent mitochondrial activity with a mean cytotoxic concentration (CC50) of 124.036 μg/ml and a low cytotoxicity was observed in hemolytic activity. Since MIB promoted less than 10% hemolysis at the highest concentration tested (800 μg/ml), the mean hemolytic concentration could not be determined. Subsequently, in vitro antioxidant assays were performed to evaluate the effects of MIB on the formation of reactive thiobarbituric acid (TBARS), nitrite (NO2-) and hydroxyl radical (HO-) species. In all assays, MIB showed equipotent antioxidant activity when compared to the positive control. Subsequently, acute oral toxicological evaluation in vivo was performed using the Fixed Dose Test (OECD Guideline 420, 2000), with modifications. No deaths or changes were observed in the clinical and behavioral parameters evaluated, as well as in serum biochemical parameters, weight and macroscopic evaluation of organs. Ahead, the ex vivo responsiveness of aortic artery preparations to acetylcholine (ACh) and sodium nitroprusside (SPN) after subacute treatment (7 days) with MIB (25, 50 and 100 mg/kg) in rats subjected was evaluated. L-NAME-induced hypertension (50 mg/kg·day for 21 days). Aortic preparations of animals treated with MIB at 25 mg/kg showed higher reactivity to acetylcholine, but did not intensify the vessel response to SNP compared to the hypertensive control group, prompting the need for further trials. In the in vivo antioxidant assays, the effects of MIB (25, 50 and 100 mg/kg) on malondialdehyde (MDA), reduced glutathione (GSH), myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase levels were investigated. (CAT) in erythrocytes or plasma of rats submitted to LNAME-induced hypertension (50 mg/kg · day for 21 days). Test results demonstrate that there was a significant reduction in MDA and MPO levels and a significant increase in GSH and SOD levels of hypertensive animals treated with MIB compared with hypertensive control. All these results underscore the immense therapeutic potential of MIB against hypertensive diseases and related oxidative stress, paving the way for the study of new therapeutic branches in the treatment of hypertensive individuals. Instituição: Universidade Federal do Delta do Parnaíba Tipo: Dissertação

2019-11-04T00:00:00Z Pinto, Jocélia do Carmo http://dspace.ufdpar.edu.br/jspui/handle/prefix/347 2023-08-18T13:18:31Z 2018-04-06T00:00:00Z

Título: Estudo fitoquímico e atividade biológica de folhas de copernicia prunifera (mill.) h.E. Moore Autor(es): Pinto, Jocélia do Carmo Primeiro Orientador: Andrade, Ivanilza Moreira de Abstract: Copernícia prunifera (Mill) H. E. Moore is a native palm tree from Brazil hat adds values both for the local economy and countrys economy, Among the various parts of the plant, the epieuticular wax that covers the surface of the leaves is the main raw material used in the manufacture of wax, used in various industrial sectors. Although widely used, there are still few phytochemical and biological studies about the species. The objective of this study was to compare two treatments for the removal of epicuticular wax (EC), ehloroform (CHCI3) and hexane (C6HI2), and characterize phytochemistry and evaluate the biological activity of aqueous, ethanolic and methanolic extracts of leaves and epicuticular wax, both without treatment and treatment of C. prunifera. The samples were collected in the city of Caxingó, Piauí Brazil. For the phytochemical characterization were used qualitative tests (phenols, tannins, saponins, reducing sugar, organic acids, polysaccharides, alkaloids and Fflavonoids) and quantitative (yield and total). For the identification of the main chemical groupings of the samples, the electrochemistry and Fourier transform infrared spectroscopy (FIIR) were made. The evaluation of the antioxidant capacity was determined by the 2,2-azinobis (3-ethyIbenzihiazoline-6-sulfonic) - ABTS + » method. The in vitro extotoxic potential of the epicuticular wax extracts was verified in four tumor lines and the biocompatibility was evaluated by analyzing the hemolytic activity on human erythrocytes, The efficacy of the two treatments was observed using surface electron microscopy (SEM). The crude percentage vield (RPB) of the aqueous extract of the mature leaves epicuticular wax (EAFM) was higher (1.9%) than the aqueous extract f the epicuticular wax of the young leaves EAFJ (1.5%). The leaves treated with chloroform had higher RPB than leaves treated with hexane. The hydrogenation potential (pH) of EC extracts were slightly acidic, 5.7 (EAFI) and 6.0 (EAEM), as well as ethanolic (4.7) and methanolic (5.9) extracts of green leaves treated with chloroform, however all other extracts of the leaves of both treatments presented neutral pH. Condensed tannins, saponins and alkaline reducing sugars and flavonols were detected in leaf extracts and cpicuticular wax. FTIR analysis revealed the presence of bands associated with these metabolites. The total phenolic content of aqueous extracts of wax was lower in EAFJ (56.48 + 21.91 mg EAG / g extract) than EAFM (158.52 + 3927 mg EAG / g extract). The antioxidant activity (AA) was 349 + 4,8 ug and 100.3 4 15.2 ug for EAFJ and EAFM, respectively. The extractions (aqueous, ethanolic and methanolic) of mature leaves treated with chloroform had higher AA when compared to mature leaves treated with hexane. Young leaves treated with hexane showed more efficient Aqueous extracts of epicuticular wax showed cytotoxicity to the glioblastoma line (SF-295), while extracts of wax and leaves treated with hexane showed low toxicity to human erythrocytes. The results obtained show that in addition to the industrial applications, the epicuticular wax and the leaves of C. prunifera present great biological potential. Instituição: Universidade Federal do Delta do Parnaíba Tipo: Dissertação

2018-04-06T00:00:00Z Silva, Priscila Costa http://dspace.ufdpar.edu.br/jspui/handle/prefix/346 2023-08-18T13:18:06Z 2021-03-23T00:00:00Z

Título: Predição in sílico da afinidade do nebivolol a proteínas envolvidas na infecção por sars-cov-2, através de um estudo de docagem molecular. Autor(es): Silva, Priscila Costa Primeiro Orientador: Medeiros, Jand Venes Rolim Abstract: In December 2019, a new coronavirus (CoV) was determined to be responsible for an outbreak of potentially fatal atypical pneumonia, defined as coronavirus-19 disease (COVID-19), caused by severe acute coronavirus 2 respiratory syndrome (SARS-CoV -2) in Wuhan, China. So far, there are no really effective drugs for such infections, making the development of drug therapies of paramount importance. A faster and more effective option in this regard is the study for the use of drugs that have already been approved for use in humans, known as drug repositioning. In this work, nebivolol hydrochloride (NEB), a third generation drug, beta-adrenoceptor antagonist, selective β1, with vasodilating and antioxidant properties, was used in the treatment of hypertension, acting directly on the renin-angiotensin system (RAS). The aim of this study was to analyze the affinity of nebivolol on the proteins that are involved, from viral entry to replication, through a molecular docking study. For this, the following procedures were carried out: obtaining the three-dimensional structures of proteins in the Protein Data Bank (PDB); survey of data related to the ligand and its three-dimensional structure by Drugbank, optimization of these structures and finally, the study of molecular docking, using computer programs such as AutodockTools, LigPlot and Pymol. The result of molecular docking showed that there was interaction between all complexes formed (ligand / protein), with binding energies equal to -9.48 kcal.mol-1 for protein S in the closed state (6VXX), -7.06 kcal / mol-1 in the open state (6VYB), -7.32 kcal / mol-1 for Mpro (6Y2E), -5.46 kcal / mol- 1 for ADAM-17 (2DDF), -5.45 kcal.mol-1 for cathepsin L (2XU3), - 5.26 kcal.mol-1 for TMPRSS2 (2OQ5), -4.94 kcal.mol-1 for the RBD domain (6LZG), and -4.84 kcal.mol- 1 for ECA2 (1R42). The best energies obtained were with protein S and Mpro, thus indicating that the ligand may be acting both in viral entry and in the replication process. Additional studies should be carried out to elucidate the antiviral role of nebivolol, however this initial virtual approach already points to possible targets and mechanisms used by nebivolol, since compared to some drugs used in the clinic, NEB demonstrated better energy. Therefore, the evaluation of the affinity of this ligand to the targets in question is indispensable for the development of therapeutic strategies against the new coronavirus. Instituição: Universidade Federal do Delta do Parnaíba Tipo: Dissertação

2021-03-23T00:00:00Z Bittencourt, Cleiton Barroso http://dspace.ufdpar.edu.br/jspui/handle/prefix/342 2023-08-16T14:29:00Z 2019-06-24T00:00:00Z

Título: Estudo de diversidade genética em populações naturais de Pilocarpus spp. da região nordeste setentrional por meio de marcadores ISSR e RAPD Autor(es): Bittencourt, Cleiton Barroso Primeiro Orientador: Andrade, Ivanilza Moreira de Abstract: Pilocarpus Vahl, popularly known as jaborandi, is a neotropical genus with distribution in Central and South America, having as its center of diversity the northern and northeastern region of Brazil. The group suffered intense extraction to obtain pilocarpine, alkaloid used for the treatment of primary glaucoma. Because population reduction endangers economically important species, assessing genetic diversity is paramount to creating more effective conservation strategies. The objective was to evaluate genetic diversity in natural populations of Pilocarpus spp. located in the Northeast Region by molecular markers ISSR and RAPD. Seven populations of jaborandi were sampled from the states of Ceará, Maranhão and Piauí of the following species: Pilocarpus demerarae Sandwith, Pilocarpus jaborandi Holmes e Pilocarpus microphyllus Stapf ex Holm. 10 ISSR primers and four RAPD primers were used, and with the data generated the intra, interpopulation and cluster diversity indices were estimated. The polymorphisms presented by the markers had an average of 63.90% (ISSR) and 59.75% (RAPD). The PIC (0.210), MI (2.23) and RP (4.78) results for the combined markers indicated that they are informative for study in Pilocarpus spp. The polymorphism rate within populations ranged from 21.90% to 36.19%, with avarage values of Shannon Diversity Index (I) and Expected Heterozygosity (He) low (0.129 and 0.080). The populations with the lowest values were Centro de Ramos and Mata Fresca, while the largest was Cutias, all represented by P. microphyllus. Molecular Variance Analysis (AMOVA) showed 63% of interpopulation and 37% intrapopulation variability. The PhiPT value (PhiPT = 0.631) indicated a large population structure and the dendrogram generated by UPGMA and structuring showed the formation of distinct groups. The molecular markers used were effective for the study of diversity in Pilocarpus spp and showed a differentiation between P. microphyllus, P. jaborandi and P. demerarae genotypes. All populations showed low genetic diversity, possibly resulting from anthropogenic pressures in recent decades. Given the great economic value of the group, projects are needed to conserve the areas where the populations studied are located, increase gene flow and create germplasm banks for the recovery and maintenance of diversity in Pilocarpus spp. Instituição: Universidade Federal do Delta do Parnaíba Tipo: Dissertação

2019-06-24T00:00:00Z